TABLE 2.

Screening of suppressor mutants reveals mutations within cdaS

GP1327 was cultured without xylose, which resulted in the formation of suppressor colonies. More than 80 suppressor colonies were isolated and sequenced for their P_xylcdaS regions.

Strain	Site of mutation	Mutation	Consequence
>70 suppressors without no.	xyl promoter	Alteration of XylR binding site	Constitutive cdaS expression
	cdaS allele	C121A	Q41K
GP1334	cdaS allele	C130T	L44F
GP1354	cdaS allele	G136A	E46K
GP1350	cdaS allele	C182T	A61V
GP1353	cdaS allele	C227T	A76V
GP1355	cdaS allele	C602A	P201Q