FIGURE 12.

Translational repression by NFAR1/2 and de-repression by NF45. A, A6 cells were analyzed by ribopuromycilation assay to measure translation in cells. Staining with an anti-puromycin antibody was reduced by treatment with 0.5 mm arsenite for 30 min. Scale bar, 10 μm. B, quantification of the cells in A. n = 32; ***, p < 0.001, t test. C, A6 cells expressing indicated proteins were analyzed by ribopuromycilation assay. Arrows denote transfected cells. Scale bar, 10 μm. D, quantification of anti-puromycin antibody staining of the transfectants in C. n = 30; *, p < 0.05; **, p < 0.01, Tukey-Kramer test between GFP-expressing cells (leftmost bar) and indicated cells.