FIGURE 2.

NF45 promotes RNA granule disassembly. A–C, A6 cells stably expressing RNG105-GFP (A) or G3BP-GFP (B and C) were transfected with NF45-mRFP1 (A and B) or mRFP1 (C). Arrows and arrowheads denote NF45-mRFP1- or mRFP1-transfected and untransfected cells, respectively. The cells were cultured in the absence of stressors. Scale bars, 10 μm. D, dose-dependent effect of NF45-mRFP1 expression on RNG105-GFP granule disassembly. Control, untransfected neighboring cells. E and F, quantification of RNG105-GFP or G3BP-GFP granule area. n = 35; **p < 0.01, Tukey-Kramer test.