FIGURE 9.

Binding of NF45 to NFAR2 does not affect the association between NFAR2 and RNG105 mRNP complexes. A, IP from A6 cells co-expressing NF45-mRFP1 and NFAR2-GFP, NFAR2-TD-GFP, NFAR2-TA-GFP, or GFP using the anti-GFP antibody in the presence or absence of RNase. Immunoprecipitates were immunoblotted with anti-GFP and anti-RFP antibodies. Lower bands of NF45-mRFP1 were detected in cell lysates (input) and the NFAR2-GFP immunoprecipitates, although the reasons are not known. B, IP with the anti-GFP antibody from A6 cells co-expressing RNG105-mRFP1 and NFAR2-GFP or GFP, which were additionally transfected with or without NF45-mRFP1. C, a model for the role of the GQSY and DZF domains of NFAR2 and NF45 in RNA granule assembly.