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. 2014 Jun 12;289(30):21191–21202. doi: 10.1074/jbc.M114.559773

FIGURE 1.

FIGURE 1.

A, molecular weight estimates of DinJ, YafQ(H87Q), and the DinJ-YafQ complex on size exclusion chromatography. Each sample was fractionated on a Superdex 75 10/300 size exclusion column. Chromatograms overlaid were displayed with molecular weight calibration standards. Molecular weight calibration standards are as indicated above the profile: blue dextran, 2000 kDa; conalbumin, 75 kDa; ovalbumin, 44 kDa; carbonic anhydrase, 29 kDa; ribonuclease A, 13.7 kDa, and aprotinin, 6.5 kDa. X axis, elution volume (ml); y axis, absorption at 280 nm (mAU, milliabsorbance units). B, chemical cross-linking of DinJ performed at 37 °C for a 1-h (lanes 3 and 5) or 2-h (lanes 4 and 6) incubation, in the presence of dimethyl pimelimidate (DMP) (lane 1, 0 mm; lanes 3 and 4, 10 mm; lanes 5 and 6, 20 mm), followed by SDS-PAGE. Monomeric (1mer), dimeric (2mer), trimeric (3mer), and tetrameric (4mer) species and positions of molecular weight markers (kDa) are shown.