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. 2014 Jun 12;289(30):21191–21202. doi: 10.1074/jbc.M114.559773

FIGURE 4.

FIGURE 4.

DinJ and DinJ-YafQ binding to the putative dinJ–yafQ operator in vitro. A, schematic of the dinJ–yafQ upstream region. It spans from its translation start site (SS) 0 to −69, including three overlapping palindromes: pal I (in boldface type), pal I-II (partially in boldface type), and pal II. Underlines indicate axes of symmetry of repeated sequences, and highlights in gray indicate the −10 box and −35 box in the promoter. Half-sites are boxed. B, EMSAs demonstrated that protein DinJ (35 μm), YafQ (35 μm), and the DinJ–YafQ complex (10 μm) binds to pal I, I-II, and II (0.5 μm), respectively. C, a comparison of DinJ (top) and DinJ-YafQ (bottom) binding ability to pal I by EMSA. D, a comparison of DinJ (top) and DinJ-YafQ (bottom) binding ability to pal I-II by EMSA. The concentration of DNA duplex was constant at 0.5 μm in all EMSAs. DinJ and DinJ-YafQ complex concentrations (0, 1, 2, 4, 5, 6, 8, 10, 15, and 20 μm) were titrated in C, and DinJ and DinJ-YafQ complex concentrations (0, 1, 5, and 10 μm) were titrated in D.