FIGURE 5.

EMSAs of DinJ-YafQ complexes with pal I and I-II shortening fragments. A, sequences of a series of pal I and pal II deletions or mutants are listed. B and C, pal I-II variants (0.5 μm) were incubated in the absence (−) or presence of decreasing concentrations of DinJ-YafQ complex (10, 5, and 1 μm) as indicated for EMSA. Samples were fractionated on a 6% polyacrylamide gel and visualized by ethidium bromide staining. D, similar tests for pal I deletions (0.5 μm) bound to DinJ-YafQ complex. Triangles, upshifted bands.