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. 2014 Jun 12;289(30):21191–21202. doi: 10.1074/jbc.M114.559773

FIGURE 6.

FIGURE 6.

Critical residues of DinJ for DNA recognition. A, potential surface charge of the (DinJ)2-(YafQ)2 complex with three sulfates coordinated in the RHH motif. B, a schematic diagram illustrates three sulfates coordinated with residues within the RHH motif of DinJ dimer. C, EMSAs of the DinJ-YafQ complexes carrying substitutions in the RHH domain of DinJ. Pal I-II (0.5 μm) was included in these assays in the absence (−) or presence of decreasing concentrations (10, 5, and 1 μm) of the WT DinJ-YafQ complex or the individual mutant complex (K8A/R16A, S31A/R35A, and K8A/R16A/S31A/R35A).