Figure 2.

Plasma augmented biofilms have reduced susceptibility to vancomycin in vitro. A-B) Viability of bacteria (Log₁₀ CFU/mL) within plasma augmented (MHB-10% Plasma, light bars) and non-augmented (MHB, dark bars) biofilms of a methicillin resistant (MRSA 3) and methicillin susceptible (UAMS-1) strain of S. aureus following overnight exposure to vancomycin (0, 2, 16, 128, and 1024 μg/mL). Bar graphs are indicative of the mean bacterial CFU ± SD. P-values generated via One-way ANOVA with a Dunnett’s post hoc test. C-D) SEM analysis of plasma and non-plasma augmented biofilms from the isolates listed above, following exposure to vancomycin. Images were captured at 2,500X. Size bars represent 10 μm.