Table 2.

Primer information containing primer name, amplified gene region, primer sequence, source of primers and PCR-product size

Combination	Primer name	Gene	Primer sequence	Source of primers	Product size (bp)
1	Tre18S_N1_for	18S rRNA	5′CCC GAC CTT CGG TGA ATC3′	this lab	~ 820
	CHtrente1.rev	18S rRNA	3′CCA CCT CCG ATC CCT AGT5′	this lab
2 (nested)	Tre18S_N2_for	18S rRNA	5′TAG GGT AGT GGC CTA CCG3′	this lab	~ 700
	CHtrente0.rev	18S rRNA	3′GTC GAG ACT ACG ACG GT5′	this lab
3	Tre18S_N5a_for	18S rRNA	5′TAG CAT GGG ATG ACA CGA TAG GA3′	this lab	~ 760
	CHtrente2.rev	18S rRNA	3′ACA AAG CTC TAG CCC CAT CA5′	this lab
4 (nested)	Tre18S_N5_for	18S rRNA	5′GGA TGA CAC GAT AGG ACT TCG3′	this lab	~ 750
	CHtrente2.rev	18S rRNA	3′ACA AAG CTC TAG CCC CAT CA5′	this lab
5	CHtrente1.for	ITS	5′ACT AGG GAT CGG AGG TGG3′	this lab	~ 700
	ITS4	ITS	3′TCC TCC GCT TAT TGA TAT GC5′	White et al. (1990)
6	CHtrente2.for	ITS	5′TGA TGG GGC TAG AGC TTT GT3′	this lab	~ 900
	ITS4	ITS	3′TCC TCC GCT TAT TGA TAT GC5′	White et al. (1990)