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. 2014 Jun 3;13:139. doi: 10.1186/1476-4598-13-139

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Copyright © 2014 Wang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunohistochemistry showed the expression of Notch1 and HES1 in cisplatin sensitive group and insensitive group. The histopathology revealed the irregular nuclei, some spindle cells, and even destroyed bone (A and B, Column 1). Immunohistochemical examination of Notch1 and HES1 were carried out in different patients. There is significant more expression of Notch1 and HES1 in cisplatin sensitive patients than cisplatin insensitive patients in gross appearance (A and B, Column 2 and 3) and semi-quantitative scatter plot below (C and D). Column 4 in A and B was the negative control with the secondary antibody alone without primary antibody. (**P < 0.01).