Table 4.
The characteristics of ASNase II-loaded CSNPs prepared by CS/TPP 0.4%/0.095% ( w / v ) and loaded with different amounts of lyophilized ASNase II
| Lyophilized protein (mg) | Size (nm) | PDI | Zeta potential (mV) | Protein content (mg) | EE (%) | Yeild (mg) | LC (%) |
|---|---|---|---|---|---|---|---|
| 1 |
250 ± 11 |
0.48 |
+35.5 ± 2 |
0.701 ± 0.011 |
70.1 |
3.02 |
23.3 |
| 2 |
262 ± 10 |
0.38 |
+30.7 ± 2 |
1.464 ± 0.05 |
73.2 |
4.18 |
35.1 |
| 3 |
295 ± 9 |
0.27 |
+24.1 ± 3 |
2.244 ± 0.105 |
74.8 |
5.5 |
40.8 |
| 4 |
340 ± 12 |
0.42 |
+21.2 ± 3 |
3.048 ± 0.07 |
76.2 |
6.4 |
47.6 |
| 5 | ND | ND | ND | ND | ND | ND | ND |
PDI < 5 and unimodal size distribution. ND, not determined (the physicochemical characteristic of the nanoparticles prepared from 5 mg of protein was not suitable for further study); data shown are the mean ± standard deviation.