Figure 5.

Effects of the electroacupuncture on the aromatase mRNA expressions by RT-PCR analysis. The upper picture shows the gel electrophoresis of the RT-PCR products for the aromatase. Total RNA fractions were isolated from the SA adipose and liver tissues of the INT, INT+EA, OVX and OVX+EA rats, and the cDNAs were prepared using standard techniques, as described in materials and methods. The RT-PCR products for aromatase were fractionated by electrophoresis through 2% agarose gels. Densitometric analysis of the mRNA concentration using aromatase/β-actin expressed as the mean with SEM bar (n_INT = 12, n_{INT + EA} = 12, n_OVX = 12 and n_{OVX + EA} = 10) in each column indicated in the lower panel. * p < 0.05 vs INT and INT+EA, # p < 0.05 vs OVX.