Fig. 2.

Schematic of the cloning process using the QIAGEN BioRobot 8000. Each step in the cloning strategy is indicated. Blue type denotes steps that are completely automated, and red type indicates steps that require some manual input. Procedures of QIAGEN-based protocols were modified, including Qiaquick Purification and DNA Mini-Prep protocols. However, most have been designed in the NESG Protein Production laboratory. A more detailed description of the robotic cloning procedure, as well as the automated protocols are provided elsewhere (Acton et al., 2005).