Table 2.

Buffers used for NMR buffer optimization screening.

Buffer ID	PH	Recipe
MJ001	6.5	20 mM MES†, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT‡, 0.02% NaN3, protease inhibitor# 1x, 10% D2O
MJ002	5.5	20 mM NH4OAc, 100 mM NaCl, 5 mM CaCl2, 10mM DTT, 0.02% NaN3, protease inhibitor 1x, 10% D2O
MJ003	4.5	20mM NH4OAc, 100mM NaCl, 5mM CaCl2, 10mM DTT, 0.02% NaN3, protease inhibitor 1x, 10% D2O
MJ004	5.0	50 mM NH4OAc, 10 mM DTT, 50 mM Arginine, 0.02% NaN3, protease inhibitor 1x, 10% D2O
MJ005	5.0	50 mM NH4OAc, 10 mM DTT, 5% CH3CN, 0.02% NaN3, protease inhibitor 1x, 10%D2O
MJ006	6.0	50 mM MES, 10 mM DTT, 50 mM Arginine, 0.02% NaN3, protease inhibitor 1x, 10%D2O
MJ007	6.0	50 mM MES, 10 mM DTT, 5% CH3CN, 0.02% NaN3, protease inhibitor 1x, 10% D2O
MJ008	6.5	25 mM Na2PO4, 450 mM NaCl, 10 mM DTT, 20 mM ZnSO4, protease inhibitor 1x, 0.01% NaN3 (pH 6.5), 10% D2O
MJ009	6.5	20 mM MES, 100 mM NaCl, 5% CH3CN, 10mM DTT, 0.02% NaN3, protease inhibitor 1x, 10% D2O
MJ010	6.5	20 mM MES, 100 mM NaCl, 50 mM Arginine, 10mM DTT, 0.02% NaN3, protease inhibitor 1x, 10% D2O
MJ011	6.5	20 mM MES, 100 mM NaCl, 1% Zwitter§, 10 mM DTT, 0.02% NaN3, 10% D2O
MJ012	6.5	20 mM MES, 100 mM NaCl, 50 mM ZnSO4, 10 mM DTT, 0.02% NaN3, protease inhibitor 1x, 10% D2O

^‡DTT: Dithiothreitol,

^†MES: 2-(N-morpholino)ethanesulfonic acid,

^§Zwitter: ZWITTERAGENT^® 3–12 Detergent cat. 963015 (CALBIOCHEM),

^¶TCEP: tris(2-carboxyethyl)phosphine,

^**Tris: tris(hydroxymethyl)aminomethane,

^#Protease inhibitor: Protease inhibitor cocktail tablets cat. 11836170001 (ROCHE).