Figure 1.

NK cell metabolism is regulated developmentally and following activation (a) Representative flow cytometric analyses of the expression of CD71 and CD98 or the amount of 2-NBDG staining from bone marrow (BM) or splenic NK CD11b^lo versus CD27^lo subsets as indicated. The bar graph shows averaged MFI (+/− s.d.) for CD71, CD98 or 2-NBDG of BM and splenic NK cell subsets (n=4 mice in 4 independent experiments, t-test, *p<0.05, **p<0.01, ***p<0.001). MFI were normalized to the CD11b^lo subset in the BM. (b) Histograms represent CD71, CD98 and 2-NBDG expression on splenic NK cells from control injected or mice injected with poly(I:C) 18 h before, one representative experiment out of 3 is shown. (c and d) Primary NK cells were stimulated or not with IL-15 for 18 h or 120 h and NK cell metabolism was analyzed. OXPHOS (OCR for O₂ Consumption Rate) (c) and Aerobic Glycolysis (ECAR for ExtraCellular Acidification Rate) (d) were analyzed in real time after injection of Glucose 25 mM, Oligomycin 1 μM, FCCP 1.5 M + pyruvate 1 mM and Actimycin A 1 μM + Rotenone 0.1 μM as indicated. Curves represent the average of 3 independent mice performed in triplicate (2 independent experiments).