Figure 3.

GADD45G Is Not Compatible with Megakaryocyte-Erythroid Differentiation and Thereby Selects for Lymphomyeloid Lineages

(A) TdTOMATO⁺ megakaryocytes after a 7 day culture of 100 LT-HSCs. Transduction efficiency was equal for both conditions. n = 3 experiments.

(B) Colony formation assay of 200 LT-HSCs transduced with GADD45G or control under permissive cytokine conditions. n = 3 experiments.

(C) Experimental scheme of (D).

(D) Transplantation of transduced LSKs in lethally irradiated recipients (four mice per condition) and BM FACS after 11 days. Transduction efficiency was 59% (GADD45G) and 72% (control).

(E) Example pedigrees illustrating the filiation of continuously tracked LT-HSCs (at the apex, generation 1) and all their progeny in subsequent generations.

(F) Percentage of pedigrees with megakaryocyte development.

(G) Percentage of cell-death events in each generation, before cells have differentiated into GMP-like cells (CD16/32⁺). Analyzed cell numbers are indicated.

(H and I) Colony formation assay of 400 MEPs or 200 GMPs transduced with GADD45G or control. n = 3 experiments.

Data are represented as mean ± SD. ^∗p < 0.05, ^∗∗p < 0.01. See also Movie S1. See also Figure S3.