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. 2014 Jun 19;3(1):34–43. doi: 10.1016/j.stemcr.2014.05.010

Figure 4.

Figure 4

GADD45G Induces Differentiation by Specifically Activating MAPK p38

(A–D) Five day culture of transduced LT-HSCs in the presence of p38 (A and B) or JNK inhibitors (C and D). FACS for immature HSPCs (A and C) and mature GM cells (B and D). n = 3 experiments.

(E) Positive control for JNK inhibitor activity by reduced NIH 3T3 expansion. n = 3 experiments.

(F and G) (F) FACS histogram and quantification of phosphorylated p38 and (G) phosphorylated MK2 in 5 day-cultured MPPs after GADD45G or control transduction by phosphoflow cytometry. MFI, mean fluorescence intensity (n = 3 experiments).

(H and I) Five day culture of transduced LT-HSCs in the absence/presence of p38 inhibitor Vx702. FACS for immature HSPCs (H) and mature GM cells (I). n = 4 experiments.

ca, constitutively active; M3K4, MAP3K4. Stars above inhibitor conditions indicate significance versus respective DMSO control. Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01. ns, not significant. See also Figure S4.