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. 2014 May 29;3(1):131–141. doi: 10.1016/j.stemcr.2014.04.017

Figure 1.

Figure 1

Characterization of Human Peripheral Blood Monocyte-Derived iPSCs

(A) Representative image of iPSC colonies in bright field. Scale bar, 200 μm.

(B) Representative images of iPSC colonies after immunostaining for the pluripotency markers NANOG, SSEA-4, OCT-4, and TRA-1-81. The merged images additionally include a DAPI staining for DNA to show that the transcription factors and pluripotency markers NANOG and OCT-4 are localized within the nuclei of the cells. Scale bars, 100 μm.

(C) Pluripotency gene expression levels as revealed by quantitative real-time PCR. Gene expression levels of undifferentiated hiPSCs (gray bars) are very similar to those of the undifferentiated human H9 cells (black bars). hB53 controls (Co) (uninfected blood mononuclear cells) show practically no pluripotency gene expression levels (white bars) (n = 3 independent experiments). Error bars, SD.