Figure 7.

Rapid Electrical Stimulation Reduces Ca²⁺ Transients and Cav1.2 Expression in hiPSC CM

(A) Representative Ca²⁺ transients recorded using fluo-4-labeled hiPSC CMs unpaced (CTRL) and paced at 1 or 4 Hz for 8 hr. Note the abnormal secondary Ca²⁺ release during decay phase (inset).

(B) Bar graphs showing magnitude of Ca²⁺ transient expressed as F/F₀ and rate of transient decay (tau, ms). ^∗p < 0.0001 compared to 1 Hz and CTRL. n = 10 independent recordings, each group.

(C) Cav1.2 expression, measured by RT-qPCR, in hiPSC CMs subjected to 1 and 4 Hz electrical stimulation.