hESC-Heps Express the Essential HCV Entry Factors
(A) Immunostaining of occludin, CD81, SR-BI, and claudin-1 in hESCs and hESC-Heps. Scale bar, 100 μm.
(B) Western blotting for HCV entry factors (occludin, CD81, SR-BI, and claudin-1), stem cell (OCT4), and cell differentiation (AFP) markers in Huh7, hESCs, and hESC-Heps, respectively.
(C) The expression level of HCV entry factors in hESC-Heps and Huh7, relative to hESC, was determined by qPCR.
Error bars represent the SD of the mean. n = 3, biological replicates. See also Tables S1 and S2.