Induction of Innate Immune Response in hESC-Heps by IL-29
(A) hESC-Heps were stimulated with different concentrations of IL-29. Proteins were extracted, and phosphorylated STAT1 and loading control, β-actin, were detected by western blotting.
(B) hESC-Heps or Huh7 cells were treated with 100 ng/ml IL-29 for the different times as indicated. Total RNA was isolated and reverse transcribed, and then qPCR was performed for the named genes.
Error bars represent the SD of the mean. n = 3, biological replicates. See also Tables S1 and S2.