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. Author manuscript; available in PMC: 2014 Jul 25.
Published in final edited form as: Methods Enzymol. 2014;540:55–72. doi: 10.1016/B978-0-12-397924-7.00004-2

Figure 2.

Figure 2

Production of the WAVE/Abi/HSPC300 trimer. (A) Sequence of the pMal-Tev vector used for all trimer constructs. (B) SDS-PAGE gel showing affinity purification of MBP-Abi158 by amylose beads. Other trimer proteins are purified similarly. (C) Anion exchange chromatography of the reconstituted WAVE217/Abi158/HSPC300 trimer. Coomassie brilliant blue stained SDS-PAGE analysis of the indicated fractions is shown below.