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. Author manuscript; available in PMC: 2014 Jul 25.
Published in final edited form as: Methods Enzymol. 2014;540:55–72. doi: 10.1016/B978-0-12-397924-7.00004-2

Figure 3.

Figure 3

Production of the Sra1/Nap1 dimer. (A) Modification of the pFastBacHTa vector to enhance expression of dimer proteins by inserting an L21 sequence. (B) Coomassie brilliant blue stained SDS-PAGE analysis following the co-purification of His6-tagged Sra1 and His6-tagged Nap1 by Ni-NTA beads from co-lysed cells. In this case, Nap1 is in excess. (C) Chromatogram of SOURCE 15Q anion exchange purification of the Sra1/Nap1 dimer. Coomassie brilliant blue stained SDS-PAGE analysis of indicated fractions is shown below.