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. Author manuscript; available in PMC: 2015 Apr 8.
Published in final edited form as: Structure. 2014 Feb 27;22(4):515–525. doi: 10.1016/j.str.2014.01.010

Figure 4.

Figure 4

Field dependent 19F CPMG relaxation dispersion data for Trp102 (squares), Trp187 (circles), and Trp203 (triangles) of 5-F-Trp labeled Ud NS1A ED dimer (600 μM) in low salt pH 8 buffer. Transverse relaxation rates (R2) as a function of νCPMG acquired at magnetic field strengths of 11.7 T (470.18 MHz) and 14.1 T (564.62 MHz) are shown in red and cyan, respectively. The relaxation dispersion curves for Trp187 at both fields were fit to a two-state exchange model (Carver and Richards, 1972),yielding the following results: kex = 6430 ± 1180 s−1; R20 (470.18 MHz) = 269 ± 18 s−1; R20 (564.62 MHz) = 328 ± 16 s−1; α = 2.3 ± 0.6.