Table 1.
19F T1 and T2 Relaxation Data for 5-F-Trp Residues in Ud NS1A ED, [K110A] NS1A ED, and [C116S,G183C] NS1A EDa
| Sample | Peak | T1 (s) | T2 (ms) |
|---|---|---|---|
| NS1A ED (600 μM) | W102 | 0.58 (0.06) | 4.51 (0.41) |
| W187 dimer | 0.63 (0.06) | 3.34 (0.16) | |
| W203 | 0.61 (0.10) | 5.44 (0.59) | |
| NS1A ED (250 μM) | W102 | 0.49 (0.07) | 5.67 (1.09) |
| W187 dimer | 0.75 (0.26) | 4.0 (1.2) | |
| W187 monomer | 0.71 (0.11) | 11.76 (3.02) | |
| W203 | 0.52 (0.06) | 5.86 (1.29) | |
| [K110A] NS1A ED (200 μM) | W102 | 0.35 (0.04) | 9.83 (2.23) |
| W187 monomer | 0.65 (0.14) | 27.06(5.89) | |
| W203 | 0.42(0.05) | 10.71 (2.14) | |
| [C116S,G183C] NS1A ED (200 μM) | W102 | 0.62 (0.05) | 3.16 (0.32) |
| W187 | 0.65 (0.06) | 4.42 (0.56) | |
| W203 | 0.68 (0.07) | 3.84 (0.55) |
a
19F relaxation datashown were obtainedat 470.18 MHz in low salt pH 8 bufferand at 20 °C. All T2 data shown correspond to a νCPMG of 2,500 Hz. Standard errors representing 95% confidence bounds for the exponential fits are given in parentheses.