Fig. 2.

Maturation of DC in vitro. Expression of maturation markers by peripheral blood DC of normal donors and cancer patients was analyzed by flow cytometry. Leukocytes in the whole blood were stained with antibody cocktails soon after blood drawing or after overnight incubation in the medium. DC expression of maturation markers, CD80, CD83, and CD86, was analyzed and compared between fresh and overnight-incubated cells. Percentages of positive cells and mean fluorescence intensity (in the parenthesis) are shown in each histogram. Representative cases are shown for normal donors (a) and cancer patients (b)