Figure 5. Monocultures containing a mostly hepatocytes (95%) or co-cultures containing NPCs in defined proportions were seeded into the reactors.

After allowing the cultures to stabilize for 3 days, dexamethasone was removed from the medium and 24 h later, 50% the wells were stimulated with 1 µg/ml LPS and/or the test drugs (ranitidine (800 mg/ml), perhexiline (5 mmol), famotidine (175 mg/ml) or DMSO controls). Measurements of LDH release were made from the medium 48 h after treatment. LPS markedly increased toxicity of the known idiosyncratic drug ranitidine but not its corresponding negative control famotidine. Dash, Griffith and Tannenbaum, publication in progress.

LDH: Dehydrogenase: LPS: Lipopolysachcharide: NPC: Non-parenchymal cell.