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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: Nature. 2014 Mar 19;507(7493):448–454. doi: 10.1038/nature13163

Extended Data Figure 10. REST, longevity and stress resistance.

Extended Data Figure 10

a. Prefrontal cortical (upper panel), hippocampal CA1 (middle panel) and cerebellar (lower panel) sections of individuals ranging in age from 57–102 years were double-labeled for REST (green) and the neuronal marker MAP2 (red). Cases with extreme longevity (98, 102 years) are associated with marked induction of REST in prefrontal cortical and hippocampal CA1 neurons, but not in cerebellar Purkinje cell (ovals) or granule cell neurons (to the right of the dashed line). Scale bar: 20 μm. b. REST increases the expression of genes associated with stress resistance and longevity. Shown are fold changes in mRNA levels for catalase (CAT), superoxide dismutase (SOD1) and FOXO1a (FOXO1) following REST knockdown (sh-RESTa or sh-RESTb) or overexpression (REST). Values represent the mean±S.D., *P<0.05 by Student’s unpaired t-test. n=3. c. FOXO1a expression is dependent on REST. Western blotting of SH-SY5Y cells shows that shRNA-mediated REST knockdown (sh-REST +) almost completely abolishes FOXO1a protein, which is prevented by shRNA-resistant mouse REST.