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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: Nature. 2014 Mar 19;507(7493):448–454. doi: 10.1038/nature13163

Figure 3. REST is neuroprotective.

Figure 3

a. REST-deficient cortical neurons cultured from REST cKO mouse embryos show increased vulnerability to H2O2 and oligomeric Aβ42 toxicity. Cell viability is expressed as percent of the value in untreated cultures. Values represent the mean ± S.E.M., n=6–8. *P<0.05, **P<0.01 by Student’s unpaired t-test. b. Reduced nuclear translocation of the ΔREST mutant. c. REST cKO mice exhibit age-related neurodegeneration. Shown are sections of the hippocampal CA1 subfield from 8 month old REST cKO mice labeled for TUNEL, cleaved caspase 3 (cl-CASP3) and GFAP, or stained with hematoxylin and eosin (H&E). d. Neurodegeneration in 8 months but not 1 month old REST cKO mice in the cortex, and in hippocampal CA1 and dentate gyrus (DG) regions. Values represent the mean ± S.E.M., n=6 mice; **P<0.001 relative to control by Student’s unpaired t-test. Scale bars: 20 μm.