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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: Nature. 2014 Mar 19;507(7493):448–454. doi: 10.1038/nature13163

Extended Data Figure 3. REST regulates expression of genes related to cell death and the neuropathology of AD.

Extended Data Figure 3

a. REST knockdown with either of two distinct shRNAs (sh-RESTa and sh-RESTb) significantly increase mRNA expression of ChIP-seq targets related to cell death and AD pathology, whereas REST overexpression (REST) represses gene expression. Values are the mean ± S.D., n=3. *P<0.05 by Student’s unpaired t-test. b. Shown are Western blots of lysates from SH-SY5Y cells transduced with a control lentiviral vector (CTRL), or 3 separate REST shRNAs (sh-REST; each lane represents a different shRNA) or REST cDNA (REST) to knockdown or overexpress REST, respectively. c. REST knockdown (sh-REST) markedly induces phosho-tau epitopes (PHF-1 and AT8). Induction of these epitopes is blocked by lithium chloride (LiCl), an inhibitor of the tau kinase GSK3β.