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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Nat Protoc. 2014 Jun 26;9(7):1740–1759. doi: 10.1038/nprot.2014.121

Figure 3. Gel size-selection of long Full-length cDNA.

Figure 3

The same agarose gel is shown under UV stained with SYBR Safe and visible light (left and right respectively). Ladder stands for the 1kb plus DNA ladder (Life Technologies; cat. no. 10787-018), LB, for gel loading buffer (containing xylene cyanol and bromophenol blue), and FlcDNA for the purified Full-length cDNA. At least one empty well is left between samples. The recommended fraction to be excised is indicated.