Table 2.

Chimera control configurations.

Configuration A-A	3′end-AAAAAAAAAAAAAAAABNNNNNNNTCTAGCGGCCGCACTGCACTCTGAGCAATACC-5′end
Configuration B-B	3′end-AAAAAAAAAAAAAAAABNNNNNNNAGATGCGGCCGCTGATCACTCTGAGCAATACC-5′end
Configuration A-B (chimera)	3′end-AAAAAAAAAAAAAAAABNNNNNNNTCTAGCGGCCGCTGATCACTCTGAGCAATACC-5′end
Configuration B-A(chimera)	3′end-AAAAAAAAAAAAAAAABNNNNNNNAGATGCGGCCGCACTGCACTCTGAGCAATACC-5′end

3′end and 5′end correspond to the regions where the cDNA sequence will be present. Random barcodes are identified in bold, 3′ and 5′ chimera barcode underlined (left and right respectively), NotI recognition sequence in italics, and the poly(A) tail and common sequence ligated to the 5′ RNA end in regular font (left and right respectivelly). Sequences are represented in 5′ to 3′orientation.