|
| ||
| Component | Amount (μL) | Final |
|
| ||
| 60 μg total RNA | 35 | 1.2 μg μL-1 |
| Capped IVT controls (1x, 500 pg μL-1, see Box 1) | 6 | 10 pg μL-1 |
| Turbo DNase buffer 10x | 5 | 1x |
| Turbo DNase enzyme (2 U μL-1) | 3 | 6 U |
| RNasin plus | 1 | |
|
| ||
| Total | 50 | |
|
| ||
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