|
| ||
| Component | Amount (μL) | Final |
|
| ||
| sticky-ends full-length cDNA | 50 | 1 ng μL -1 or less |
| water | 470 | |
| T4 DNA ligase buffer (10x) | 60 | 1x |
| T4 DNA ligase (2000 U μL-1) | 20 | 66.6 U μL -1 |
|
| ||
| Total | 600 | |
|
| ||
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