|
| ||
| Component | Amount (μL) | Final |
|
| ||
| Sample beads | 20.5 | |
| Quick ligation Buffer (2x) | 25 | 1x |
| Barcoded linkers (1 μM) | 0.5 | 10 pM |
| T4 DNA ligase (2000 U μL-1) | 3 | 120 U μL-1 |
|
| ||
| Total | Approx. 50 | |
|
| ||
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