|
| ||
| Component | Amount (μL) | Final |
|
| ||
| Sample beads | 20 | |
| Water | 4 | |
| Phusion High-Fidelity PCR Master-Mix (2x) | 25 | 1x |
| Illumina oligo PE1.0 (5 μM) | 0.5 | 0.1 μM |
| Illumina oligo PE2.0 (5 μM) | 0.5 | 0.1 μM |
|
| ||
| Total | 50 | |
|
| ||
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