|
| ||
| Component | Amount (μL) | Final |
|
| ||
| Linearized DNA template (200ng) | 22.5 | |
| Transcription Optimized 5x buffer | 10 | 1x |
| DTT (0.1M) | 5 | 10 mM |
| NTP mix (2.5 mM each) | 10 | 0.5 mM |
| T3 RNA polymerase (10 U μL-1) | 2 | 0.4 U μL-1 |
| RNasin Plus | 0.5 | - |
|
| ||
| Total | 50 | |
|
| ||
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.