|
| ||
| Component | Amount (μL) | Final |
|
| ||
| pooled IVTs (1μg) | 14.5 | |
| capping buffer (10x) | 2 | 1x |
| GTP (10 mM) | 1 | 0.5 mM |
| SAM (2 mM freshly diluted from the 32mM stock) | 1 | 0.1 mM |
| Vaccinia Capping Enzyme (10 U μL-1) | 1 | 0.5 U μL-1 |
| RNasin Plus | 0.5 | - |
|
| ||
| Total | 20 | |
|
| ||
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