|
| |||
| Cycle number | Denature | Anneal | Extend |
|
| |||
| 1 | 98°C, 30 s | ||
| 2-11 | 98°C, 20 s | 60°C, 30 s | 72°C, 5 min (adding 10 sec / cycle) |
| 12 | 72°C, 5 min | ||
|
| |||
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.