|
| ||
| Component | Amount (μL) | Final |
|
| ||
| Phosphatase treated sample | 16.3 | - |
| TAP reaction buffer (10x) | 2 | 1x |
| DTT (0.1 M) | 0.2 | 1 mM |
| Tobacco Acid Pyrophosphatase (TAP, 10 U μl-1) | 0.5 | 5 U |
| RNasin plus | 1 | - |
|
| ||
| Total | 20 | |
|
| ||
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