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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Nat Protoc. 2014 Jun 26;9(7):1740–1759. doi: 10.1038/nprot.2014.121
Component Amount (μL) Final
TAP treated sample 4.5
100 mM DNA/RNA oligo 5RNAGsuI 1 10 mM
buffer T4 RNA Ligase 1 (10x, containing 10 mM ATP) 1 1x
T4 RNA Ligase 1 (10 U μl-1) 2 20 U
DMSO 1 10% (vol/vol)
RNasin plus 0.5 -
Total 10