| ||
Component | Amount (μL) | Final |
| ||
Total ligated RNA | 10 | - |
PET-3RT oligo (1 μM) (Tube A only) or PET-3RT-B (1 μM) (Tube B only) | 1 | 83 nM |
dNTPs (10 mM) | 1 | 0.83 mM |
| ||
Total | 12 | |
|
| ||
Component | Amount (μL) | Final |
| ||
Total ligated RNA | 10 | - |
PET-3RT oligo (1 μM) (Tube A only) or PET-3RT-B (1 μM) (Tube B only) | 1 | 83 nM |
dNTPs (10 mM) | 1 | 0.83 mM |
| ||
Total | 12 | |
|