|
| ||
| Component | Amount (μL) | Final |
|
| ||
| cDNA template | 5 | |
| Water | 18 | - |
| Phusion High-Fidelity PCR Master-Mix (2x) | 25 | 1x |
| forward oligo (5 μM) ( PET-5ABio for tube 1 or PET-5BBio for tube 2) | 1 | 0.1 μM |
| reverse oligo (5 μM) ( PET-3A for tube 1 and PET-3B for tube 2) | 1 | 0.1 μM |
|
| ||
| Total | 25 | |
|
| ||
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