|
| |||
| Cycle number | Denature | Anneal | Extend |
|
| |||
| 1 | 98°C, 30 s | ||
| 2-11 | 98°C, 20 s | 50°C, 30 s (adding 1°C/cycle) | 72°C, 5 min (adding 10 sec / cycle) |
| 12 | 72°C, 5 min | ||
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