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. Author manuscript; available in PMC: 2014 Jul 25.
Published in final edited form as: Adv Funct Mater. 2011 Nov 17;22(2):239–260. doi: 10.1002/adfm.201101707

Table 3.

NO-releasing materials for the inhibition of neointimal hyperplasia.

Authors Ref. Animal model Application NO source Delivery system NO detection method NO release or flux Time point in vivo I/M ratio decrease
Kaul et al. [122] Rat Periadventitially in balloon-injured ileofemoral arteries SPER/NO Atrigel Chemiluminescence Peak at 4.5 μmol (40 min) 95% released (150 min) 14 d ~75%
Pearce et al. [123] Rat Perivascularly in balloon-injured carotid arteries PAN/NO PROLI/NO Poloxamer N/A N/A 14 d 86% (PROLI/NO powder)
Kapadia et al. [126] Rat Perivascularly in balloon-injured carotid arteries PROLI/NO DPTA/NO Peptide amphiphile and heparin (nanofiber gels) Griess Peak at 0.6 μmol day−1 and 1.2 μmol day−1, respectively (2 d) 14 d 77% and 45%, respectively
Lipke et al. [37] Rat Periadventitially in balloon-injured carotid arteries Cys/NO PEG Griess (HEPES buffer) 35% released (4 h)[127] 14 d 77% compared to control hydrogel
Serrano et al. [39] Rat Perivascularly balloon-injured carotid arteries diamine diol/N2O2 POC, PDDC Griess Peak flux 35.4 · 10−9 mol cm−2 min−1 (4 h) and 6.6 · 10−9 mol cm−2 min−1 (24 h), respectively 14 d 38%
Do et al. [129] Rabbit Microsphere-loaded stent deployed in infrarenal abdominal aorta NOC-12/N2O2 PLGA and PEG (microspheres) Griess ~18 mM (1 week) 7 d & 28 d 46% and 32%, respectively, compared to control microspheres
~8 mm (4 weeks)
Yoon et al. [130] Porcine Coated stent deployed in coronary arteries SNP PU Famotidine assay to measure SNP (no specified temp) 85% of total SNP mass eluted from stents (30 d) 28 d No improvement
Buergler et al. [131] Porcine Coated stent deployed in left anterior descending and right coronary arteries DETA/NO Polycaprolactone Chemiluminescence (flushed with He, no specified temp and buffer) 9.0 · 10−5 μmol min−1 mg−1 (0 h) and 0.5 · 10−5 μmol min−1 mg−1 (72 h) 28 d No improvement
Abbasi et al. [132] Rabbit Perivascularly in aortic intimal injured hyper-cholesterolemic rabbits DETA/NO Silastic elastomer N/A N/A 6 wks No improvement

Note: Time points for NO release or flux are the same as the in vivo time points unless otherwise specified. NO detection was conducted at 37 °C in PBS. I/M ratio decrease is compared to injury alone unless otherwise specified.

Cys/NO: S-nitrosocysteine, DETA/NO: diazeniumdiolated diethylenetriamine, DPTA/NO: 1-[N-(3-aminopropyl)-N-(3-ammoniopropyl] diazen-1-ium-1,2-diolate, I/M: Intima/media, NOC-12/N2O2: diazeniumdiolated N-ethyl-2-(1-ethyl-2-hydroxy-2-nitrosohydrazino)ethanamine, PAN/NO: diazeniumdiolated poly(acrylonitrile), PDDC: poly(1,12-dodecanediol-co-citrate), PEG: poly(ethylene glycol), PLGA: poly(lactic-co-glycolic acid), POC: poly(1,8-octanediol-co-citrate), PROLI/NO: 1-[2-(carboxylato)pyrrolidin-1-yl]diazen-1-ium-1,2-diolate, PU: polyurethane, SNP: sodium nitroprusside, SPER/NO: diazeniumdiolated spermine.