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. 2014 Jul 25;9(7):e102014. doi: 10.1371/journal.pone.0102014

Figure 3. Effect of CR blocking antibody on the cytokine production and activation of caspase-1 of monocytes infected in vitro with DENV-2.

Figure 3

(A) The specific scatter gate using the combination of side scatter and forward scatter, followed by the percentage using quadrant statistics applied on FL5/PECy5.5 (CD14) versus FL2/PE (CD11c) dot-plot distributions for caspase-1 analysis. Representative FAM FLICA Caspase 1 marker was quantified using the percentage determined by histogram distribution plots from analysis of the monocytes that were non-infected (NI), infected and infected but pre-treated with CR3 (CD11b/CD18) blocking antibody. Supernatant concentrations of TNF-α (B) and IFN-α (C) were quantified by CBA and ELISA, respectively, after DENV-2 infection of monocytes. The mean percentage reduction of both cytokines when cultures were pre-treated with CR blocking antibody is shown (C). The Wilcoxon-test was used in order to analyse the differences among the various conditions. Statistically significant p-values for differences between patients and controls are shown above the pairs.