Table 2. Weight % nitrogen (%N), weight % carbon (%C), % yield of gelatin relative to undemineralised bone starting weight (% collagen), and Raman organic-phosphate ratio [C-H/PO4 3− (ν 1)] for eight samples for which stable isotope analysis indicates weight % nitrogen at extremely low levels (7 µg or less), including one sample with extremely low weight % carbon.
Raman ID | Burial no. | Archaeological phase | % nitrogen w/w | % carbon w/w | % collagen | Raman ratio |
1 | 560 | Neolithic | 0.11 | 2.11 | 1.88 | - |
4 | 80 | Neolithic | - | 3.43 | 0.12 | 0.189 |
6 | 146 | Bronze | - | 6.76 | 0.27 | 0.150 |
7 | 189 | Bronze | 2.74 | 9.28 | 6.00 | - |
10 | 532 | Bronze | - | 3.44 | 0.60 | 0.150 |
11 | 550 | Bronze | - | 1.04 | 1.75 | 0.247 |
31 | 479 | Iron | - | - | 0.16 | 0.208 |
32 | 516 | Iron | - | 3.72 | 0.40 | 0.295 |
37 | 329 | Iron | - | 1.85 | 0.26 | 0.147 |
39 | 481 | Iron | - | 1.27 | 0.79 | 0.145 |
Two further samples included here (burials 189 and 560) could not have their Raman ratio determined due to fluorescence. The %N, %C, and the atomic CN ratio [Atomic C:N = (%C/%N) ×1.1666] from stable isotope analysis are provided to illustrate mass spectrometry indicators of protein survival. The % collagen yield is calculated from bone weight before demineralisation.