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. 2014 Jul 25;9(7):e98462. doi: 10.1371/journal.pone.0098462

Table 2. Weight % nitrogen (%N), weight % carbon (%C), % yield of gelatin relative to undemineralised bone starting weight (% collagen), and Raman organic-phosphate ratio [C-H/PO4 3− (ν 1)] for eight samples for which stable isotope analysis indicates weight % nitrogen at extremely low levels (7 µg or less), including one sample with extremely low weight % carbon.

Raman ID Burial no. Archaeological phase % nitrogen w/w % carbon w/w % collagen Raman ratio
1 560 Neolithic 0.11 2.11 1.88 -
4 80 Neolithic - 3.43 0.12 0.189
6 146 Bronze - 6.76 0.27 0.150
7 189 Bronze 2.74 9.28 6.00 -
10 532 Bronze - 3.44 0.60 0.150
11 550 Bronze - 1.04 1.75 0.247
31 479 Iron - - 0.16 0.208
32 516 Iron - 3.72 0.40 0.295
37 329 Iron - 1.85 0.26 0.147
39 481 Iron - 1.27 0.79 0.145

Two further samples included here (burials 189 and 560) could not have their Raman ratio determined due to fluorescence. The %N, %C, and the atomic CN ratio [Atomic C:N  =  (%C/%N) ×1.1666] from stable isotope analysis are provided to illustrate mass spectrometry indicators of protein survival. The % collagen yield is calculated from bone weight before demineralisation.