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. 2014 Jul 25;9(7):e102340. doi: 10.1371/journal.pone.0102340

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© 2014 Ilyas et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Proteins (150 µg) extracted from T. asahii were labelled with 0.2 mM IAF and subjected to 1DE. The gels were scanned for IAF fluorescence in a Typhoon scanner followed by staining in colloidal coomassie. Panel A shows coomassie-stained gels whilst panel B is the corresponding IAF fluorescence image. Changes in total spot intensity between treated and untreated cells were quantified by Image Quant software analysis (Panel C).