Table 2. Identification of T. asahii yeast proteins.
| Spot | Identified Protein | Organism | Accession number d | MW m | Protein Scorea/Confidence Interval (%) | Ion Score/Confidence Interval (%) | Sequence Coverage (%) | No. of MS/MS patterns assigned to peptidesb | P-value | Fold change |
| Metabolic enzymes (energy and respiration) | ||||||||||
| 1 | 3-Isopropylmalate dehydrogenase | Trichosporon asahii var. asahii CBS 8904 | gi|406698071 | 53569 | 694/100 | 639/100 | 44 | 6 | 6.43E-04 | 1.6 |
| 2 | Phospholipase B | Trichosporon asahii var. asahii CBS 2479 | gi|401884039 | 71965 | 870/100 | 837/100 | 27 | 7 | 0.002 | 1.6 |
| 3 | Malate dehydrogenase | Trichosporon asahii var. asahii CBS 2479 | gi|401884576 | 35270 | 1190/100 | 1133/100 | 67 | 8 | 0.005 | −2 |
| 4 | 3-Hydroxyisobutyryl- CoA hydrolase | Trichosporon asahii var. asahii CBS 8904 | gi|406696953 | 57118 | 1190/100 | 1073/100 | 61 | 8 | 0.005 | −2.2 |
| 5 | Aconitase | Trichosporon asahii var. asahii CBS 8904 | gi|406694125 | 85654 | 1320/100 | 1228/100 | 36 | 9 | 0.007 | −2.5 |
| 6 | Aconitase | Trichosporon asahii var. asahii CBS 8904 | gi|406694125 | 85654 | 1320/100 | 1228/100 | 36 | 9 | 0.008 | −1.6 |
| 7 | Homoserine dehydrogenase Hom6 | Trichosporon asahii var. asahii CBS 8904 | gi|406695321 | 40376 | 338/100 | 303/100 | 28 | 2 | 0.017 | −1.7 |
| 8 | Alanine-glyoxylate aminotransferase | Trichosporon asahii var. asahii CBS 2479 | gi|401885827 | 51027 | 891/100 | 815/100 | 45 | 7 | 0.017 | 2 |
| 9 | Aldehyde reductase I (Alcohol dehydrogenase) | Trichosporon asahii var. asahii CBS 2479 | gi|401886852 | 64647 | 1560/100 | 1479/100 | 50 | 10 | 0.02 | −1.9 |
| 10 | ATP synthase alpha chain, precursor | Trichosporon asahii var. asahii CBS 2479 | gi|401889058 | 58090 | 1170/100 | 1068/100 | 47 | 9 | 0.023 | 1.7 |
| 11 | Aconitase | Trichosporon asahii var. asahii CBS 8904 | gi|406694125/ | 85654 | 799/100 | 712/100 | 35 | 5 | 0.015 | −2 |
| 12 | Phosphoglycerate kinase | Trichosporon asahii var. asahii CBS 8904 | gi|406694335 | 49924 | 1890/100 | 1734/100 | 61 | 10 | 0.025 | −1.5 |
| Protein biosynthesis and folding | ||||||||||
| 13 | Translation elongation factor 2 | Trichosporon asahii var. asahii CBS 8904 | gi|406694343 | 51124 | 423/100 | 397/100 | 36 | 4 | 0.009 | −3.4 |
| 14 | Endoribonuclease LPSP | Aeromonas hydrophila subsp. hydrophila ATCC 7966 | gi|117619955 | 13357 | 199/100 | 186/100 | 46 | 1 | 4.45E-05 | 2.3 |
| Nonfunctional and abnormal protein degradation | ||||||||||
| 15 | 20S Proteasome beta-type subunit, Pre3p | Trichosporon asahii var. asahii CBS 8904 | gi|406698063 | 25360 | 435/100 | 422/100 | 33 | 4 | 0.036 | 1.8 |
| Protein repair and folding | ||||||||||
| 16 | Heat shock protein | Trichosporon asahii var. asahii CBS 2479 | gi|401888774 | 71440 | 548/100 | 490/100 | 39 | 4 | 0.002 | −3.1 |
| 17 | Heat shock protein | Trichosporon asahii var. asahii CBS 8904 | gi|406695586 | 98932 | 1200/100 | 1065/100 | 42 | 7 | 0.019 | −3.1 |
| 18 | Heat shock protein 70 | Trichosporon asahii var. asahii CBS 2479 | gi|401882131 | 70187 | 846/100 | 798/100 | 34 | 8 | 0.021 | −4.5 |
| 19 | Heat shock protein70 | Trichosporon asahii var. asahii CBS 2479 | gi|401882131 | 70187 | 1960/100 | 1704/100 | 63 | 11 | 0.034 | −2.1 |
| Hypothetical Proteins | ||||||||||
| 20 | Hypothetical protein A1Q1_08001 | Trichosporon asahii var. asahii CBS 2479 | gi|401886770 | 21217 | 1310/100 | 1266/100 | 53 | 9 | 3.44E-05 | 2.5 |
| 21 | Hypothetical protein A1Q2_03020 | Trichosporon asahii var. asahii CBS 8904 | gi|406699588 | 60263 | 441/100 | 403/100 | 35 | 2 | 0.011 | −2.4 |
| 22 | Hypothetical protein A1Q1_06950 | Trichosporon asahii var. asahii CBS 2479 | gi|401887835 | 33117 | 432/100 | 399/100 | 30 | 3 | 0.011 | 1.9 |
| 23 | Hypothetical protein A1Q1_06913 | Trichosporon asahii var. asahii CBS 2479 | gi|401887891 | 71289 | 1460/100 | 1403/100 | 38 | 11 | 0.011 | 1.8 |
| 24 | Hypothetical protein A1Q1_00754 | Trichosporon asahii var. asahii CBS 2479 | gi|401888905 | 53663 | 539/100 | 503/100 | 42 | 4 | 0.013 | −1.6 |
| 25 | Hypothetical protein A1Q2_04745 | Trichosporon asahii var. asahii CBS 8904 | gi|406697616 | 53697 | 333/100 | 333/100 | 20 | 2 | 0.016 | −2.1 |
| 26 | Hypothetical protein A1Q2_04745 | Trichosporon asahii var. asahii CBS 8904 | gi|406697616 | 53697 | 341/100 | 341/100 | 7 | 2 | 0.034 | −1.9 |
| 27 | Hypothetical protein A1Q1_06913 | Trichosporon asahii var. asahii CBS 2479 | gi|401887891 | 71289 | 964/100 | 904/100 | 38 | 8 | 0.041 | 1.6 |
Protein thiols were labeled with IAF following exposure to NaAsO2 and identified by MALDI-TOF/TOF MS. MW (molecular masses) are expressed in Da. Spots (1–12) showed up-regulated metabolic enzymes; (16–19) were involved in protein synthesis and repair enzymes; (20–31) were hypothetical proteins. Spots taken arbitrarily showed NA p-value and fold-change. These all proved to be hypothetical proteins.
a
Protein score probability limit (where P<0.05) was 86.
b
Peptides with confidence interval >95% were considered.
d
Database accession number obtained from NCBI (top hits shown).
m
mass of identified proteins.